Technological advancement in medicine has helped come up with better diagnostic methods. The cardiac Elisa kits are the latest invention in this field. They are enzyme-dependent test devices that help in determining the presence or absence of heart diseases. These equipments are capable of discerning problems in hearts of virtually all animals.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This test can also be used in detecting foreign bodies that exist in low concentrations. Heart problems can, therefore, be identified before they become chronic. The patient is advantaged; he will spend less money fighting a developing problem than he would have spent on a chronic one. This is because it is cheaper treating a disease while still in its early stages than when it has developed into a complex illness.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
For this experiment to work, one needs to prepare all samples, standards and reagents. He/she should then add a small amount of the sample to every well and then incubate for about two hours. After this, he should aspire then add some reagent and again incubate for about an hour. After this, he must aspire the mixture and wash it three times. The next step is addition of substrate solutions and then incubating for another 20 to 25 minutes. The last step is addition of stop solution and making readings.
The enzyme sandwich principle is applied in this experiment. Plates on the kits are coated in advance with specific antibodies for the problem under investigation. Standards or samples are then appropriately added to the plates. They normally contain antibodies which are specific to certain defects. Lastly, Avidin conjugate is put on each plate and then incubated.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
This process depends is an enzyme dependent process that uses color change as an indicator of reactions in reagents. The process works through an enzyme immunoassay which combines with antigens producing the subsequent color change. This test is capable of establishing the presence of both antibodies and antigens.
This test can also be used in detecting foreign bodies that exist in low concentrations. Heart problems can, therefore, be identified before they become chronic. The patient is advantaged; he will spend less money fighting a developing problem than he would have spent on a chronic one. This is because it is cheaper treating a disease while still in its early stages than when it has developed into a complex illness.
Proper working of this equipment means it is sensitive to reactions, gives accurate results, and is capable of making many detailed readings at a time. When a tool is sensitive, it can exhibit any slight change resulting from the reaction between samples and reagents. Its accuracy ensures that results obtained are free of errors, and hence, believable. They are also manufactured to work on specific problems.
Stability is also important in the working of this equipment. To ensure stability, the loss rate of the activity has to be kept as low as possible. Good storage is also very important in ensuring stability. For the purpose of minimizing the effects of the environment on this experiment, standard lab conditions are very important; room temperature, standard pressure and humidity. The temperatures within the incubator should be closely regulated. It is also important to have one person working on the experiment from beginning to end.
For this experiment to work, one needs to prepare all samples, standards and reagents. He/she should then add a small amount of the sample to every well and then incubate for about two hours. After this, he should aspire then add some reagent and again incubate for about an hour. After this, he must aspire the mixture and wash it three times. The next step is addition of substrate solutions and then incubating for another 20 to 25 minutes. The last step is addition of stop solution and making readings.
The enzyme sandwich principle is applied in this experiment. Plates on the kits are coated in advance with specific antibodies for the problem under investigation. Standards or samples are then appropriately added to the plates. They normally contain antibodies which are specific to certain defects. Lastly, Avidin conjugate is put on each plate and then incubated.
After the addition of substrate solutions, it is only the wells that will contain type 3 of Tropin I. The reagents will then show a change in color. The reaction is brought to an end by adding a solution of sulphuric acid, and the change in color is measured is a special style.
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